10/30/2022 0 Comments Biorad western blot protocolInsufficient protein may have been loaded on the gel.Incorrect storage of antibodies or ECL western blotting detection reagents may result in a loss of signal.Check protein transfer by staining the gel and/or membrane. Transfer efficiency may have been poor.Run the required positive control with your samples to confirm. If low signal or no signal is detected, make sure that the phosphorylation state of the protein was appropriately induced.If high background is observed, replace milk with 5% (w/v) BSA in TBS-T for blocking and antibody dilution as phosphor-specific antibody detects casein in the milk.Add phosphatase inhibitors and keep samples on ice at all times to preserve the phosphorylated state of the proteins.The blocking and incubation agents used were not freshly prepared or were too dilute.Post-antibody washes may not have been performed for a sufficient period of time or were not performed in a high enough volume.Contamination can be transferred to the blots from electrophoresis and related equipment used in blot preparation. Transfer buffers may have become contaminated.Exposures can vary from 5 seconds to 60 minutes. Place the wrapped blots, protein side up, in an X-ray film cassette and expose to x-ray film.Drain off the excess detection reagent, wrap up the blots, and gently smooth out any air bubbles.The final volume required is 0.125ml/cm 2. Incubate membrane (protein side up) with 10ml of ECL (enhanced chemiluminescence substrate) for 1-2 minutes. Wash 4 times for 10 minutes each with TBS containing 0.1% Tween-20 and once for 2 minutes with PBS.Incubate the membrane for 30 minutes at room temperature with horseradish peroxidase (HRP)- conjugated secondary antibody, diluted to 1:1000 - 1:5000 in 5% nonfat dry milk/ TBS-T.Wash three times for 5 minutes each with Wash Buffer (TBS containing 0.1% Tween-20).Place the membrane in the primary antibody solution and incubate for 2 hours at room temperature, or overnight at 4☌ with agitation. Dilute the primary antibody to the recommended concentration/dilution in 5% nonfat dry milk/TBS-T.Incubate the blot for 1 hour at room temperature, or overnight at 4☌ with agitation.Remove the blotted membrane from the transfer apparatus and immediately place in blocking buffer consisting of 5% nonfat dry milk/TBS-T**.After boiling, continue as normal to the membrane blocking step of the protocol. Immediately after transferring the gel onto the membrane, submerge the membrane in boiling PBS for 5 minutes. Transfer the proteins to a nitrocellulose or PVDF membrane with variable power settings according to the manufacturer’s instructions.įor Amyloid Beta Detection, Boiling Method: Set gel running conditions according to the manufacturer’s instructions.*Guidelines for choosing gel percentages are based on protein size to be detected: 4-5% gel, > 200 kD 7.5% gel, 120-200 kD 8-10% gel, 40-120 kD 13% gel, 15-40 kD 15% gel, < 20 kD. Load up to 40µl of sample to each well of a 1.5mm thick gel*.Remove 20µl of supernatant and mix with 20µl of 2x sample buffer. Transfer the supernatant to a new tube and discard the pellet.Lyse the cell pellet with 100µl of lysis buffer on ice for 30 min (For 1 X 10 6 cells, lyse with 100µl of lysis buffer).Place cells in a microcentrifuge tube and centrifuge to collect the cell pellet.10X TBS-T (Tris-buffered saline containing Tween-20): Dissolve 80g of NaCl, 2g of KCl, 30g of Tris base and 10ml Tween-20 in 800ml of distilled H 2O.Blotting Membrane: Nitrocellulose or PVDF membrane.Alternate Primary and Secondary Antibody Dilution Buffer: 1X TBS-T with 4% Bovine Serum Albumin (BSA).Alternate Blocking Buffer: 1X TBS-T with 4% Bovine Serum Albumin (BSA).Primary and Secondary Antibody Dilution Buffer: 1X TBS-T with 5% nonfat dry milk.Blocking Buffer: 1X TBS-T with 5% nonfat dry milk.Transfer Buffer: 3.0g Tris base, 14.4g Glycine, 200ml Methanol.
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10/30/2022 0 Comments Gundry sanitarium#GUNDRY SANITARIUM FREE#The Enoch Pratt Free Library took charge of the small “library station” and, with strong support from neighborhood residents, opened a small Colonial Revival branch library in 1951. The effort grew quickly and the organizers asked the developers of Edmondson Village Shopping Center to donate a space for the community. In 1943, a group of local residents from Ten Hills and Edmondson Village came together to start a neighborhood lending library. Enoch Pratt Free Library Edmondson Avenue Branch Courtesy Baltimore County Public Library. In recent decades, the Edmondson Village Shopping Center has struggled with competition from larger suburban shopping malls, but still remains an important anchor for nearby neighborhoods. A bowling alley arrived in 1949 and the Hecht Company opened a store across the street in 1958. For decades after WWII, shoppers enjoyed over 29 shops selling everything from fur coats to auto supplies to ice cream. The Baltimore Evening Sun called the project “a suburban shopping center of harmonious design, said to be unique in American city planning.” Architects Kenneth Miller and James Edmunds combined traditional Colonial Revival design with innovative features including a terraced 500-car parking lot that made the shopping center one of first designed for drivers. Jacob and Joseph Meyerhoff, 1950 Courtesy Baltimore County Public Library.īuilt by developers Jacob and Joseph Meyerhoff, the Edmondson Village Shopping Center opened to huge crowds on May 7, 1947. “Small wonder that so many thousands of Baltimoreans make it a point of pride to bring out-of-town visitors to Edmondson Village, a landmark of Baltimore progress.” Capturing this story, Edmondson Village boasts landmarks from sites that date from when the area was more country than city up to landmarks from heyday of mid-century Baltimore. Residents fought for new schools and libraries. Shoppers filled the parking lots at brand-new shopping centers. In the years after WWII, Baltimore could hardly keep up with a quickly growing number of houses and people. Explore stories of an innovative shopping center, a treasured community library, and the famed Edmondson-Westside High School.
Stefano created a Style Sheet that you could use as a starting point for your projects. But styles are very important for keeping your layout consistent, applying changes effectively, or using features like Table of Contents or Hyperlinks. If you haven’t mastered them already, you should learn them ASAP (check out our tutorial on InDesign Paragraph Styles).Īlthough paragraph styles are very practical, help you with creating a better layout and save lots of time, many InDesign users don’t use them – even when they know them and they understand the advantages.Ĭreating all the styles in a document might be a huge effort and sometimes it might look like it makes little sense. Integrating this feature can improve your productivity tremendously and reduce the time you spend on laying out your documents. Selecting a region changes the language and/or content on Styles are the single most useful feature in InDesign. csv and txt field in the above mentioned format with a # sign on their column name. To generate QR codes, follow these instructions: I have a multi- page document with lots of separate tables on each page. Email: MATMSG:\nTo: (example: Business Card: "BEGIN:VCARD\nVERSION:2.1\nN:Smith John\nFN:John Smith\nORG:Adobe\nTITLE:Engineer\nTEL CELL: 919876543210\nTEL WORK VOICE:123456789\nADR WORK: Street the data entries can be a mix of email, sms, hyperlink, or plain text type. To import your Excel spreadsheet into an InDesign document, first open your.Web Hyperlink: URL: (For example “URL:”). INDESIGN DATA MERGE MULTIPLE LINES IN ONE CELL HOW TOIn this video, you will learn how to quickly generate multiple business. INDESIGN DATA MERGE MULTIPLE LINES IN ONE CELL CODEPlain Text: In the QR code Column enter the text as it is. Data Merge is a brilliant time-saver feature in InDesign that allows you to automate and batch create variations on a layout.csv files, which are used as Data Source, are in the following format: The source file contains the data and images that will. To generate the QR codes the data entries in the. InDesign will datamerge into one document, whereas Illustrator is a little. You can do this task by following the step-by-step guide below. Following are the different types of QR code fields that can be added via a Data Merge workflow: It seems that inDesign loads the file (no errors whatsoever), but then it does not. Merging cells in Word involves combining two adjacent cells of similar size into one larger cell. Solution: when merging, in the Options tab select fit frame to images, and then replace all of your blank icon cells with a 1×1 pixel image called blank.png. You can integrate a QR code in the merged document. The merged document is the resulting InDesign document that contains the boilerplate information from the target document, repeated as many times as it takes to accommodate each record from the data source. INDESIGN DATA MERGE MULTIPLE LINES IN ONE CELL PLUS
10/30/2022 0 Comments Resume star statements examples#Resume star statements examples how to#Related: How To Use Keywords and Phrases in Your Resume and Cover Letter (With Tips and Examples) Words to include in a power statement #Resume star statements examples software#Additionally, as many companies are moving towards software for tracking and sorting through resumes, power statements can help you include the keywords and phrases that you need to pass the screening. Power statements and power words tend to stand out to those who are reviewing resumes because of their value, so using them may get your resume noticed more easily. Since power statements require you to be specific, including them can show your impact more effectively. For those who might feel shy about their accomplishments, setting a goal to write power statements can allow you to explain things factually without feeling uncomfortable. Power statements highlight professional accomplishments and personal attributes. Related: A Complete Resume Summary Guide (40+ Examples) Benefits of a power statementĪ power statement is beneficial on a resume because it shows prospective employers what you are capable of in a positive and concise way. Power words are positive and should relate directly to the skills and abilities you have. The base of any power statement is a power word, which might include an action verb, keywords from your industry, popular skills, attributes you value about yourself or values from a prospective employer's website. Companies that review your resume should be able to see from your power statements what you are capable of and what you have achieved for other employers. A power statement is usually concise and descriptive. You might use a power statement in your summary or objective, or in your descriptions of responsibilities and achievements from previous positions. What is a power statement?Ī power statement is a phrase or sentence you include on your resume to show prospective employers the types of skills you have. In this article, we define what a power statement is, describe the benefits of using a power statement, list a variety of power words and explain how to write a power statement. When used effectively, power statements can help you get hired for the job you want. Power statements can be used in your summary or objective, in your previous job descriptions or even in your cover letter. Power statements can be quite helpful in creating strong and compelling job application materials. 10/30/2022 0 Comments Rockler router bit center depth gaugeRockler has 37 retail locations across 20 states – plus 60 independent reseller locations nationwide, as well as extensive catalog and internet operations. Photos of the Rockler Router Bit Center Depth Gauge can be viewed via this Dropbox link: Ĭelebrating its 65th anniversary as a family-run business, Rockler Woodworking and Hardware is the nation’s premier supplier of specialty hardware, tools, lumber and other high-quality woodworking products. #ROCKLER ROUTER BIT CENTER DEPTH GAUGE FREE#For store locations or a free catalog, visit or call 1-877-ROCKLER. The Rockler Router Bit Center Depth Gauge (55010) is priced at $19.99 and can be purchased exclusively online at or at Rockler Woodworking and Hardware stores nationwide, as well as through the Rockler catalog. Up close look at the center of the Router Bit Center Depth Gauge adjusted to the. The head of the gauge also includes a flat "depth" section for measuring from the edge or the top of a cutter, allowing the gauge to be used to set bit or blade cutting height. I have a rockler router table that didnt come.with accessories. Holding the gauge against the bit shank, the user adjusts the router table fence until it's tight against the broad foot at the bottom of the ruler and locks down the fence. SETUP ROUTER BITS ACCURATELY-set and depth of your router with great accuracy and precision EXCELLENT QUALITY AND DURABILITY- handy for woodworking- solid. Then the user moves the gauge up to the bit, fitting the correct half-round slot – 1/4" or 1/2" – tight to the bit's shank. To set up a router table for cutting grooves or dadoes, the user slides the clear beveled indicator to the desired metric or imperial mark on the 12" rigid steel ruler and locks in the setting by tightening the knurled knob. "The Rockler Router Bit Center Depth Gauge allows you to do both quickly and accurately." "Setting the distance from the fence and the cutting height of the bit are two of the most important operations when routing grooves or flutes," said Steve Krohmer, Rockler's vice president for product development. The gauge also can be used to precisely set the cutting height of router bits and saw blades. The Rockler Router Bit Center Depth Gauge ( ) enables users to quickly set the fence on a table-mounted router – or an edge guide on a handheld router – an exact distance from the center of the bit, which is essential when routing dadoes, grooves or flutes. Its offset foot helps the gauge avoid the bit opening in the fence.Rockler Woodworking and Hardware has developed a tool to help make it quicker and easier to achieve pinpoint accuracy in common router setup operations. For example, if you wish to cut a groove, just measure from the center of the groove to the edge of the board, and set the gauge to that distance. The tool’s fence niftily registers off the center of a bit via notches that slip around bits with either 1⁄4-in. Just set the hairline indicator for the distance you need from the fence to the center of the bit, hook it onto the bit shank, then slide the fence over until it touches the gauge. Adjusting the router table’s fence is where this gauge really shines, though. The broad foot at the end of the scale makes the tool stable. To set or measure the cutter height, simply set the movable fence, lock it, and stand the gauge over the bit. The gauge works just as accurately for measuring the height of cutters. shanks.įlat area works well for setting cutter height. The notches allow the scale to register off the center of bits with either 1⁄4-in. The gauge’s moveable fence, which has a hairline cursor, slides smoothly and locks in place firmly. steel rule etched clearly with both metric and imperial measurements. Rockler’s new Router Bit Center Depth Gauge is a clever product that makes router table fence and bit adjustments a snap. 10/30/2022 0 Comments Imeme alternativeThe book preserves visions of the divine feminine that may be shocking to some, inspirational to others. The book’s myths, legends, songs, and overheard divine conversations range from the sixth century to the twentieth and carry the potential to shake the foundation of our enduring ideas, or memes if you will, about gender and religion. Our new anthology- A Garland of Forgotten Goddesses: Tales of the Feminine Divine from India and Beyond-offers readers just such an opportunity. It can be refreshing to delve into alternative conceptions of the divine and what it means to lead a spiritual life. These religious imaginings of gender-to say nothing of race or class-have real consequences that many of us live with every day. Some theologians may retort that God’s gender doesn’t matter, but then why not default to feminine for a change? When God is conceived as male, what does that mean for women and people of alternative gender identities? It has tended to mean a subordinate existence, if not violent abjection. Yet, why would a supreme being be bound by such an algorithmic sense of coherence? Indian civilization, my area of focus, has always presented the divine as transcending such simplistic binaries.Īnother ancient meme, shared and propagated by the vast majority of the billions of religious faithful the world over, is that God is male, and that men, being made in his likeness, are the rightful rulers in families and society. One ancient meme-originating, perhaps, in the eastern Mediterranean-says that there must be either one God or many gods. 10/30/2022 0 Comments Truck repair shopLook at the potential for a walk-in client with this particular location, which means you want a place with high traffic near a freeway. If you’re looking at an actual building for your service shop, then consider location first. Invest in a good truck because, after all, you’d hate to break down on your way to serve someone else’s vehicle. The mobile shop works well if you want to get your name out there but don’t have the finances for a building yet.Ī good mobile shop will have a place for tools, fluids, and parts along with a compressor, generator, welder, floor lights, and potentially a crane. A service truck works well to service a fleet of trucks, especially if a customer doesn’t care that you’re in their yard. You also have a limited scope of skills with a mobile shop, but you can offer your services to a fleet versus the average joe looking for an oil change. Because you do not have a shop, per se, you do not have to invest yet in certain tools, like a hoist. They’re just a shop on wheels.Ī repair truck will serve customers remotely, giving them the luxury of you going to them. #TRUCK REPAIR SHOP HOW TO#Keep reading to learn how to start your own diesel truck repair shop.Īs you consider the move from diesel technician to diesel truck repair owner, you begin with one decision: truck or shop.ĭiesel repair trucks have the mobility that a shop may not, and yet they are still a legitimate truck repair shop business, without the foundation. Diesel mechanics keep trucks on the road and keep goods moving from place to place. Trucks matter so much that writer Stan Mack predicts if the trucking industry stopped for some reason or another, so would the United States economy.īehind the industry of big rigs and eighteen-wheelers reside the skilled grease monkeys that make them move. This growth only makes sense as businesses continue to grow and need diesel trucks to transport their goods from place to place in our country. The United States Bureau of Labor and Statistics estimates the field will grow 9 percent from 2016 to 2026. |
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